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nikon eclipse te2000 5 microscope  (Nikon)


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    Structured Review

    Nikon nikon eclipse te2000 5 microscope
    Nikon Eclipse Te2000 5 Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 4732 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+te2000+5+microscope/ECLIPSE+E200/pmc11127001__pnas__2318591121__sapp-16-12-12
    Average 99 stars, based on 4732 article reviews
    nikon eclipse te2000 5 microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Scalable Generation of Pre-Vascularized and Functional Human Beige Adipose Organoids.
    Article Snippet: .. Imaging of spheroid size was performed during the culture process at indicated times using a Nikon eclipse TE2000-5 microscope with a 10X objective. ..

    Article Title: Scalable Generation of Pre‐Vascularized and Functional Human Beige Adipose Organoids
    Article Snippet: .. Imaging of spheroid size was performed during the culture process at indicated times using a Nikon eclipse TE2000‐5 microscope with a 10X objective. ..

    Microscopy:

    Article Title: Scalable Generation of Pre-Vascularized and Functional Human Beige Adipose Organoids.
    Article Snippet: .. Imaging of spheroid size was performed during the culture process at indicated times using a Nikon eclipse TE2000-5 microscope with a 10X objective. ..

    Article Title: Scalable Generation of Pre‐Vascularized and Functional Human Beige Adipose Organoids
    Article Snippet: .. Imaging of spheroid size was performed during the culture process at indicated times using a Nikon eclipse TE2000‐5 microscope with a 10X objective. ..

    Article Title: Involvement of AMPA Receptor and Its Flip and Flop Isoforms in Retinal Ganglion Cell Death Following Oxygen/Glucose Deprivation.
    Article Snippet: Retinal ganglion cells were treated in conditions of RGC medium, GD, or OGD for 4 hours followed by the incubation of fura-2-AM (3 lM; #F1221; Invitrogen) for 30 minutes at 378C. .. Retinal ganglion cells then were washed with 378C normoxic or hypoxic Krebs–Ringer buffer solution (115 mM NaCl, 2.5 mM CaCl2, 1.2 mM MgCl2, 24 mM NaHCO3, 5 mM KCl, 25 mM HEPES, and 5 mM glucose, pH 7.4) The ratiometric fluorescent intensities (ex, 340 and 380 nm; em, 510 nm) of fura-2-AM were measured following the stimulation of AMPA receptors with s-AMPA (100 lM) using a Nikon Eclipse TE2000-5 microscope and the NIS-Elements AR3.2 software (Nikon Instruments, Melville, NY, USA). ..

    Article Title: Neuroprotective effects of inhibitors of Acid-Sensing ion channels (ASICs) in optic nerve crush model in rodents.
    Article Snippet: Purpose: The purpose of the current study was to assess the potential involvement of acid-sensing ion channel 1 (ASIC1) in retinal ganglion cell (RGC) death and investigate the neuroprotective effects of inhibitors of ASICs in promoting RGC survival following optic nerve crush (ONC).. Results: ASIC1 protein was significantly increased in optic nerve extracts at day 7 following ONC in rats.. Activated calpain-1 increased at 2 and 7 days following ONC as evidenced by increased degradation of αfodrin, known substrate of calpain.

    Article Title: CD44 induced enhancement of phosphatase activity and calcium influx: Modifications of EGR-1 expression and cell proliferation
    Article Snippet: .. Intracellular calcium was measured at 37 °C by the ratiometric technique using fura-2-AM (excitation at 340 nm and 380 nm, emission at 510 nm) (Invitrogen, Carlsbad, CA) as described by utilizing a Nikon Eclipse TE2000-5 microscope and NIS-Elements AR3.2 software (Nikon Instruments, Melville, NY) . ..

    Article Title: Hydroxyurea treatment inhibits proliferation of Cryptococcus neoformans in mice
    Article Snippet: .. Cells were washed with phosphate buffered saline (PBS, 50 mM, pH 7) and further suspended in PBS before analyzing them under a Nikon Eclipse (TE2000-5) microscope with a 40×/100× objective lens. ..

    Software:

    Article Title: Involvement of AMPA Receptor and Its Flip and Flop Isoforms in Retinal Ganglion Cell Death Following Oxygen/Glucose Deprivation.
    Article Snippet: Retinal ganglion cells were treated in conditions of RGC medium, GD, or OGD for 4 hours followed by the incubation of fura-2-AM (3 lM; #F1221; Invitrogen) for 30 minutes at 378C. .. Retinal ganglion cells then were washed with 378C normoxic or hypoxic Krebs–Ringer buffer solution (115 mM NaCl, 2.5 mM CaCl2, 1.2 mM MgCl2, 24 mM NaHCO3, 5 mM KCl, 25 mM HEPES, and 5 mM glucose, pH 7.4) The ratiometric fluorescent intensities (ex, 340 and 380 nm; em, 510 nm) of fura-2-AM were measured following the stimulation of AMPA receptors with s-AMPA (100 lM) using a Nikon Eclipse TE2000-5 microscope and the NIS-Elements AR3.2 software (Nikon Instruments, Melville, NY, USA). ..

    Article Title: Neuroprotective effects of inhibitors of Acid-Sensing ion channels (ASICs) in optic nerve crush model in rodents.
    Article Snippet: Purpose: The purpose of the current study was to assess the potential involvement of acid-sensing ion channel 1 (ASIC1) in retinal ganglion cell (RGC) death and investigate the neuroprotective effects of inhibitors of ASICs in promoting RGC survival following optic nerve crush (ONC).. Results: ASIC1 protein was significantly increased in optic nerve extracts at day 7 following ONC in rats.. Activated calpain-1 increased at 2 and 7 days following ONC as evidenced by increased degradation of αfodrin, known substrate of calpain.

    Article Title: CD44 induced enhancement of phosphatase activity and calcium influx: Modifications of EGR-1 expression and cell proliferation
    Article Snippet: .. Intracellular calcium was measured at 37 °C by the ratiometric technique using fura-2-AM (excitation at 340 nm and 380 nm, emission at 510 nm) (Invitrogen, Carlsbad, CA) as described by utilizing a Nikon Eclipse TE2000-5 microscope and NIS-Elements AR3.2 software (Nikon Instruments, Melville, NY) . ..

    Concentration Assay:

    Article Title: Neuroprotective effects of inhibitors of Acid-Sensing ion channels (ASICs) in optic nerve crush model in rodents.
    Article Snippet: Purpose: The purpose of the current study was to assess the potential involvement of acid-sensing ion channel 1 (ASIC1) in retinal ganglion cell (RGC) death and investigate the neuroprotective effects of inhibitors of ASICs in promoting RGC survival following optic nerve crush (ONC).. Results: ASIC1 protein was significantly increased in optic nerve extracts at day 7 following ONC in rats.. Activated calpain-1 increased at 2 and 7 days following ONC as evidenced by increased degradation of αfodrin, known substrate of calpain.

    Saline:

    Article Title: Hydroxyurea treatment inhibits proliferation of Cryptococcus neoformans in mice
    Article Snippet: .. Cells were washed with phosphate buffered saline (PBS, 50 mM, pH 7) and further suspended in PBS before analyzing them under a Nikon Eclipse (TE2000-5) microscope with a 40×/100× objective lens. ..



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    (A) Representative <t>epifluorescence</t> images (20×) of L4 animals expressing Pins-3::GFP under basal conditions (20 °C on Nematode Growth Media plates seeded with OP50), under oxidative stress (1 mM FeSO 4, 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous tyramine (15 mM). Scale bar 50 µm. (B) Corresponding quantification of fluorescence levels per worm. Scatter dot plot with relative expression of Pins-3::GFP normalized to basal conditions of each independent experiment. Line at the median. n = 50−200 animals per condition (distributed across 3–4 independent experiments). One-way ANOVA and Dunn’s post hoc test vs. basal were used. * p < 0.05, **** p < 0.0001. (C) Log 2 fold-changes in ins-3 transcript levels in animals exposed to oxidative stress (1 mM FeSO 4 , 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous Tyramine (15 mM). Negative and positive values indicate down- and up-regulation of ins-3 , respectively. Fold change was calculated as ΔCt basal conditions/ΔCt test conditions. Results are shown as mean ± s.e.m. n = 4 independent experiments. The data underlying this figure can be found at https://osf.io/wfgvs/ .
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    Image Search Results


    (A) Representative epifluorescence images (20×) of L4 animals expressing Pins-3::GFP under basal conditions (20 °C on Nematode Growth Media plates seeded with OP50), under oxidative stress (1 mM FeSO 4, 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous tyramine (15 mM). Scale bar 50 µm. (B) Corresponding quantification of fluorescence levels per worm. Scatter dot plot with relative expression of Pins-3::GFP normalized to basal conditions of each independent experiment. Line at the median. n = 50−200 animals per condition (distributed across 3–4 independent experiments). One-way ANOVA and Dunn’s post hoc test vs. basal were used. * p < 0.05, **** p < 0.0001. (C) Log 2 fold-changes in ins-3 transcript levels in animals exposed to oxidative stress (1 mM FeSO 4 , 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous Tyramine (15 mM). Negative and positive values indicate down- and up-regulation of ins-3 , respectively. Fold change was calculated as ΔCt basal conditions/ΔCt test conditions. Results are shown as mean ± s.e.m. n = 4 independent experiments. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Journal: PLOS Biology

    Article Title: The neurohormone tyramine stimulates the secretion of an insulin-like peptide from the Caenorhabditis elegans intestine to modulate the systemic stress response

    doi: 10.1371/journal.pbio.3002997

    Figure Lengend Snippet: (A) Representative epifluorescence images (20×) of L4 animals expressing Pins-3::GFP under basal conditions (20 °C on Nematode Growth Media plates seeded with OP50), under oxidative stress (1 mM FeSO 4, 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous tyramine (15 mM). Scale bar 50 µm. (B) Corresponding quantification of fluorescence levels per worm. Scatter dot plot with relative expression of Pins-3::GFP normalized to basal conditions of each independent experiment. Line at the median. n = 50−200 animals per condition (distributed across 3–4 independent experiments). One-way ANOVA and Dunn’s post hoc test vs. basal were used. * p < 0.05, **** p < 0.0001. (C) Log 2 fold-changes in ins-3 transcript levels in animals exposed to oxidative stress (1 mM FeSO 4 , 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous Tyramine (15 mM). Negative and positive values indicate down- and up-regulation of ins-3 , respectively. Fold change was calculated as ΔCt basal conditions/ΔCt test conditions. Results are shown as mean ± s.e.m. n = 4 independent experiments. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Article Snippet: For ins-3 and ins-4 expression levels analysis, animals containing the corresponding transcriptional GFP reporter ( Pins-3::GFP and Pins-4:GFP , respectively) were imaged using an epifluorescence microscope (Nikon Eclipse TE2000-5) coupled to a CCD camera (Nikon DS-Qi2) with 20× objective.

    Techniques: Expressing, Fluorescence

    Representative epifluorescence images (40×) of young not gravid adults expressing Pges-1::INS-3::VENUS in wild-type and tyra-3 null mutant background, in the absence or presence of exogenous tyramine (15 mM). Scale bar 25 µm. Fluorescence is observed in the two posterior coelomocytes of worms (inset). Right. Corresponding quantification of fluorescence intensity on the coelomocytes (normalized to wild-type animals without tyramine exposure). Line at the median. n = 35–40 animals per condition distributed across three independent experiments. For conditions with tyramine, a two-tailed Student’s t test vs. the same strain without tyramine was used. * p < 0.05. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Journal: PLOS Biology

    Article Title: The neurohormone tyramine stimulates the secretion of an insulin-like peptide from the Caenorhabditis elegans intestine to modulate the systemic stress response

    doi: 10.1371/journal.pbio.3002997

    Figure Lengend Snippet: Representative epifluorescence images (40×) of young not gravid adults expressing Pges-1::INS-3::VENUS in wild-type and tyra-3 null mutant background, in the absence or presence of exogenous tyramine (15 mM). Scale bar 25 µm. Fluorescence is observed in the two posterior coelomocytes of worms (inset). Right. Corresponding quantification of fluorescence intensity on the coelomocytes (normalized to wild-type animals without tyramine exposure). Line at the median. n = 35–40 animals per condition distributed across three independent experiments. For conditions with tyramine, a two-tailed Student’s t test vs. the same strain without tyramine was used. * p < 0.05. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Article Snippet: For ins-3 and ins-4 expression levels analysis, animals containing the corresponding transcriptional GFP reporter ( Pins-3::GFP and Pins-4:GFP , respectively) were imaged using an epifluorescence microscope (Nikon Eclipse TE2000-5) coupled to a CCD camera (Nikon DS-Qi2) with 20× objective.

    Techniques: Expressing, Mutagenesis, Fluorescence, Two Tailed Test